Li Lab Members

Feng Hong, M.D., Ph.D.

Research Assistant Professor (hongf@musc.edu)

Research Interests

  • The roles of gp96 in cancer biology and gut homeostasis
  • The Biochemistryand biology of gp96 co-chaperone

Selected Publications

  • Wu S, Dole K, Hong F, Noman AS, Issacs J, Liu B, Li Z. (2012) Chaperone gp96-independent Inhibition of Endotoxin Response by Chaperone-based Peptide Inhibitors. J Biol Chem. 2012 Jun 8;287(24):19896-903.
  • Wu S, Hong F, Gewirth D, Guo B, Liu B, Li Z. (2012) The molecular chaperone gp96/GRP94 interacts with Toll-like receptors and integrins via its C-terminal hydrophobic domain. J Biol Chem. 2012 Feb 24;287(9):6735-42.
  • Staron M, Wu S, Hong F, Stojanovic A, Du X, Bona R, Liu B and Li Z.‎ (2011) Heat shock protein gp96/grp94 is an essential chaperone for platelet glycoprotein Ib-IX-V complex. Blood. 117(26):7136-44.

Bill (Xingjun) Wu, Ph.D. 

Research Instructor (wub@musc.edu)

Research Interests

  • Mechanisms of TLR folding
  • Roles and regulations of sphingolipids in inflammation and cancer

Selected Publications

  • Novgorodov SA, Wu BX, Gudz TI, Bielwaski J, Ovchinnikova TV, Hannun YA, Obeid LM. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-COA. J. Biol. Chem. 2011 286:25352-62.
  • Wu BX, Clarke CJ, Matmati N, Bartke N, and Hannun YA. Structural requirements for selective binding of neutral sphingomyelinase 2 to anionic phospholipids, J. Biol. Chem. 2011, 286:22362-71.
  • Jenkins RW, Clarke CJ, Canals D, Snider AJ, Gault CR, Heffernan-Stroud L, Wu BX, Simbari F, Roddy P, Kitatani K, Obeid LM, Hannun YA Regulation of CC ligand 5/RANTES by acid sphingomyelinase and acid ceramidase. J. Biol. Chem. 2011, 286:13292-303.
  • Wu BX, Rajagopalan V, Roddy PL, Clarke CJ, Hannun YA. Identification and characterization of murine mitochondrial-associated neutral sphingomyelinase (MA-nSMase), the mammalian sphingomyelin phosphodiesterase 5. J. Biol. Chem. 2010, 285:17993-8002

Ephraim A. Ansa-Addo, Ph.D.

Postdoctoral Scholar (ansaaddo@musc.edu)

Research Interests

  • Determine the post-transcriptional mechanisms by which TGF-β controls T cell fate specification
  • Determine the role of TGF-β-mediated EMT genes in T cell biology

Selected Publications

  • Cestari, I., Ansa-Addo, E.A., Poliana Deolindo., Inal, J.M and Ramirez, M.I. Trypanosoma cruzi immune evasion mediated by host cell-derived microvesicles. J. Immunol. 188, 1942-52 (2012).
  • Inal, J.M., Ansa-Addo, E.A., Stratton, D., Kholia, S., Antwi-Baffour S, Jorfi S, Lange S. Microvesicles in health and disease. Invited Review - *Archivum Immunologiae et Therapiae Experimentalis* (AITE). 60, 107-21 (2012).
  • Grant, R., Ansa-Addo, E.A., Stratton, D., Antwi-Baffour, S., Jorfi, S., Kholia S., Krige L., Lange S., Inal, J.M. A filtration-based protocol to isolate Plasma Membrane-derived Vesicles andexosomes from blood plasma.J. Immunol. Methods.371, 143-51 (2011).
  • Ansa-Addo, E.A., Lange, S., Stratton, D., Antwi-Baffour, S., Cestari, I., Ramirez, M.I., McCrossan, M.V and Inal, J.M. Human Plasma Membrane-derived Vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells. J. Immunol. 185, 5236-46 (2010).

Mohammad Salem, Ph.D.

Postdoctoral Scholar (salemmo@musc.edu)

Research Interests

  • The role of regulatory T cells on immune tolerance
  • The activation process of the transforming growth factor beta (TGFβ)

Selected Publications

  • Salem M, Seidelin JB, Eickhardt S, Alhede M, Rogler G, Nielsen OH. Species-specific engagement of human NOD2 and TLR signaling upon intracellular bacterial infection: Role of Crohn’s associated NOD2 gene variants. Clin Exp Immunol. 2015, 179:426-34.
  • Pedersen J, Coskun M, Soendergaard C, Salem M, Nielsen OH. Inflammatory pathways of importance for management of inflammatory bowel disease. World J Gastroenterol. 2014, 7;20:64-77.
  • Coskun M, Salem M, Pedersen J, Nielsen OH. Involvement of JAK/STAT signaling in the pathogenesis of inflammatory bowel disease. Pharmacol Res. 2013, 76:1-8.
  • Salem M, Seidelin JB, Rogler G, Nielsen OH. Muramyl dipeptide responsive pathways in Crohn's disease: From NOD2 and beyond. Cell Mol Life Sci. 2013, 70:3391-404.
  • Salem M, Nielsen OH, Kris Nys, Yazdanyar S, Seidelin JB. Impact of T300A variant of ATG16L1 in antibacterial response and course of Crohn’s disease. Clin Transl Gastroenterol. 2015, 6, e122; doi:10.1038/ctg.2015.47.
  • Salem M, Ammitzboell M, Nys K, Nielsen OH. ATG16L1: a multifunctional susceptibility factor in Crohn’s disease. Autophagy. 2015, 3;11:585-594.

Alessandra Metelli

Student, Ph.D. Training Program (metellia@musc.edu)

Research Interests

  • Investigation of the role of gp96 and its client proteins in autoimmunity and cancer
  • Characterization of the genes required for cancer immune resistance

Selected Publications

  • Zhang, Y., Wu, B.X., Metelli, A., Thaxton, J.E., Hong, F., Rachidi, S., Ansa-Addo, E., Sun, S., Vasu, C., Yang, Y. and Liu, B., 2015. GP96 is a GARP chaperone and controls regulatory T cell functions. The Journal of clinical investigation, 125(2), pp.859-869.
  • Zhang, Y., Wu, B., Metelli, A., Hong, F., Ansa-Addo, E., Sun, S., Liu, B. and Li, Z., 2015. Molecular chaperone gp96/grp94 is critical for immunosuppressive functions of regulatory T cells (IRM15P. 458).The Journal of Immunology,194(1 Supplement), pp.199-6.

Caroline Wallace Fugle

Student, Ph.D. Training Program(wallacc@musc.edu)

Research Interests

  • Role of B cell surface TGFbeta in autoimmunity

Selected Publications

  • Metelli, A., Wu, B., Fugle, C.W., Rachidi, S., Sun, S., Zhang, Y., Wu, J., Tomlinson, S., Howe, P., Garrett-Mayer, E., Liu, B., and Li, Z. Cancer promotion and immune tolerance via surface expression of GARP. Accepted, Cancer Research.
  • Fugle, C.W., Zhang, Y., Hong, F., Sun, S., Westwater, C., Rachidi, S., Yu, H., Kirkwood, K., Liu, B., Li, Z. CD24 blunts squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells. In Press, Oncoimmunology
  • Nelson, M., Bowers, J., Bailey, S., Diven, M., Fugle, C.W., Kaiser, A., Wrzesinski, C., Liu, B., Restifo, N., Paulos, C. Toll-like receptor agonist therapy can profoundly augment the antitumor activity of adoptively transferred CD8+ T cell without host preconditioning. J Immunother Cancer 2016; 4:6.
  • Chhabra, S., Jain, S., Wallace, C., Hong, F., and Liu, B. High expression of endoplasmic reticulum chaperone grp94 is a novel molecular hallmark of malignant plasma cells in multiple myeloma. J Hematol Oncol 2015; 8:77.
  • Morales, C., Rachidi, S., Hong, F., Sun, S. Ouyang, X., Wallace, C., Zhang, Y., Garret-Mayer, E., Wu, J., Liu, B., Li, Z. Immune chaperone gp96 drives the contributions of macrophages to inflammatory colon tumorigenesis. Cancer Res 2014; 74:446-459.

Brian Riesenberg

Student, Ph.D. Training Program(riesenbe@musc.edu)

Research Interests

  • How factors of the tumor microenvironment can supress T cell imunity

Selected Publications

  • C. Bryce Johnson, Brian P Riesenberg, Bennett R. May, Stuart C. Gilreath, Guangfu Li, Kevin F Staveley-O'Carroll, Elizabeth Garrett-Mayer, Shikhar Mehrotra, David J Cole and Mark P. Rubinstein. Effector CD8+ T cell engraftment and anti-tumor immunity in lymphodepleted hosts is IL-7Rα dependent. Cancer Immunology. 2015 July 2.
  • Ansa-Addo, E., Thaxton, J., Hong, F., Wu, B., Zhang, Y.,Fugle, C.W., Metelli, A., Riesenberg, B., Williams, K., Gerwith, D., Chiosis, G., Liu, B., Li, Z.(2016). Clients and Oncogenic Roles of Molecular Chaperone gp96/grp94.Current Topics in Medicinal Chemistry,16(25), 2765–2778.

Tony Kwon

Student, Medical Scientist Training Program (kwonhyu@musc.edu)

Research Interests

  • Tumor-intrinsic immune escape mechanisms

Selected Publications

  • Fattouh R*, Kwon H*, Czuczman MA, Copeland JW, Pelletier L, Quinlan ME, Muise AM, Higgins DE, and Brumell JH. 2014. The Diaphanous-related Formins Promote Protrusion Formation and Cell-to-Cell Spread of Listeria monocytogenes. J Infect Dis. 211(7):1185-95. PMID: 25281757.
  • Roppenser B, Kwon H, Canadien V, Xu R, Devreotes PN, Grinstein S, and Brumell JH. 2013. Multiple host kinases contribute to Akt activation during Salmonella infection. PLoS One. 8(8):e71015. doi: 10.1371/journal.pone.0071015.

Karina Webb

Lab Technician (webbka@musc.edu)

Research Interests

  • Protein - protein interactions in the TLR signaling pathway