Skip to main content

CDLD Cell Models Core

Engineering advanced cellular models for the study of digestive and liver disease

The Cell Models Core provides advanced cellular models, genome engineering expertise, and training to accelerate research in digestive and liver disease. By enabling precise disease modeling, the core empowers investigators to test hypotheses and drive discovery.

Key technologies

  • CRISPR genome editing
  • iPSC differentiation
  • Organoid systems

Cell model offerings

The Cell Models Core serves as a central resource within the Center for Biomedical Research Excellence (COBRE) in Digestive & Liver Disease for developing and distributing high-quality cellular systems tailored to digestive disease research.

  • Genetically engineered cell lines
  • Pluripotent stem cell–derived liver and gut cells
  • Organoid model systems
  • Primary cells from animal models
  • Training and technical support

Capabilities

Genome Engineering

  • CRISPR-based modification of cell lines and iPSCs

  • Introduction of disease-relevant mutations

  • Generation of reporter and tagged cell lines

Stem Cell & Organoid Models

  • Differentiation of iPSCs into hepatocytes and cholangiocytes

  • Generation of gastrointestinal organoids

Primary Cell Isolation

  • Isolation of liver cells from animal models

  • Support for functional studies

Training and Expertise

  • Hands-on training

  • Access to validated reagents and protocols

  • Ongoing technical support

Impact

  • 37 genetically modified cell lines generated
  • 17 independent recipient cell lines
  • 84 research projects supported
  • 8 laboratories trained
  • 22 manuscripts produced
  • 15 grants supported
  • 1 patent and 1 spin-off company

Key Scientific Aims

Aim 1: Engineered Cell Models
Develop genetically modified models using CRISPR technology.

Aim 2: Stem Cell-Derived Systems
Generate liver, gastrointestinal cells, and organoids.

Aim 3: Primary Cell Resources
Provide isolated liver cells for mechanistic studies.

Leadership & Operations

Stephen A. Duncan, D.Phil. – Core Director
Kristen Engevik, Ph.D. – Organoid technologies
Don Rockey, M.D. – Cell isolation
Christy Kappler, Ph.D. – Operations

Why This Core Matters

The Cell Models Core enables precise disease modeling, accelerates discovery, and strengthens research capacity within the CDLD.

Next Steps

Coming soon:

  • Request Cell Models Core Services
  • Explore the Center's Research Resources
  • Collaborate with the Core's Team