Department of Regenerative Medicine and Cell Biology Equipment and Resources

RMCB Equipment and Resources

Bio-Rad ChemiDoc gel scanner
BSB 649C
Contact Antonis Kourtidis

ChemiDoc Imagers offer best-in-class performance with ease of use for visible light (RGB) and far red/near infrared (FR/NIR) fluorescence and chemiluminescence detection and all general gel documentation applications.Stain-free imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots.

For use, sign up at the Departmental Booking Scheduler with your Net ID

Agilent xCELLigence Real-Time Cell Analysis (RTCA) system
BSB 641B
Contact Antonis Kourtidis

The Agilent xCELLigence Real-Time Cell Analysis (RTCA) SP instrument uses label-free cellular impedance to continuously monitor cell health, behavior, and function with high accuracy, sensitivity, and reproducibility. The SP model can run a 96-well electronic microplate (E-Plate 96), which is ideal for cell health characterization, immune cell-mediated killing, viral cytopathic effects, cytotoxicity, cell adhesion, cell barrier function (TEER), cell signaling (e.g., GPCR) and other related applications.

For use, sign up at the Departmental Booking Scheduler with your Net ID


Two Sorvall Evolution Superspeed RC Centrifuges
BSB 634
Sign up first at BSB 621; contact Antonis Kourtidis

Accelerating your productivity with superior functionality and proven reliability. The Thermo Scientific Sorvall Superspeed Centrifuge is the perfect choice offering high speeds and RCFs.


Sorvall Discovery 90SE Ultracentrifuge
BSB 634
Sign up first at BSB 621; contact Antonis Kourtidis

The Sorvall Discovery 90SE Ultra Centrifuge features simple keypad and large LCD display for easy set-up of routine spins. A Dual display of SET and RUN conditions - verify performance at a glance. On-line rotor catalog - call up specifications for SORVALL and competitive rotors. Automatic RCF/RPM mode - no protocol conversion necessary for rotors in the catalog. Real Time Control for delayed start/stop runs.

RMCB Imaging Core

Zeiss AxioImager M.2
BSB 648
Contact Ray Deepe

Axio Imager 2 combines everything you've ever asked for in your research environment: brilliant optics and bright fluorescence. The contrast manager and light manager ensure defined conditions and reproducible results at all times. For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID.


Keyence BZ-X810
BSB 644
Contact Ray Deepe

The BZ-X Series is an all-in-one fluorescence microscope equipped with advanced observation capabilities, a fully-motorized control system for instant target acquisition, and advanced imaging and analysis functions. With a built-in darkroom and an installation area of ~ 1' x 2' the BZ-X inverted fluorescence microscope can be set up in any location for optimal testing efficiency. The high-sensitivity, cooled CCD camera can be switched between monochrome and color imaging, and supports fluorescence, brightfield, and phase contrast imaging. The large motorized stage can quickly move to the desired observation location, while high-speed auto-focusing and automatic capture conditions allow any user to easily capture publication-quality images. Additionally, the BZ-X Series all-in-one microscope is capable of three-dimensional analysis, motion analysis, and time-series quantification.


Zeiss Axio Scope A1 Microscope
BSB647
Contact Christi Kern

Axio Scope provides a range of uses which are as versatile as the tasks found in industry and research. They range from simple routine applications to more complex research projects. From quality control to material analysis and development. From surface examination and structure characterization to complex digital analysis. Equipped with a high quality 6 megapixel color camera offers you an amazingly fast live image and acquisition speed despite its large pixel count and the very large 16 mm diagonal field of view. This scope takes excellent brightfield images with DIC capabilities for the 10X and 63X objectives.


Nikon TE 2000-S
BSB 644A
Contact Ray Deepe

In early 2000, Nikon introduced their flagship inverted microscope system, the TE2000. This was the first in the now world-famous Eclipse inverted series of microscopes, which took advantage of the breakthrough CFI60 Infinity Optical System. This microscope system represented a vast leap forward in microscope technology and marked the beginning of the flagship Eclipse series of microscopes that are leading the industry in technology and market share to this day.


Olympus BX-40 Fluorescent Inverted Microscope
BSB 644B
Contact Ray Deepe

The BX series (BX40, BX50, and BX60), were launched in 1993. They use a novel Y-shaped design for the microscope body. The series featured superior operability, excellent rigidity, and first-class optical performance. Developed for routine clinical procedures, with an ergonomic design meant to offer excellent performance while helping reduce fatigue in the arms and wrists during prolonged use. For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID


Olympus IX50
BSB 644B
Contact Antonis Kourtidis

The Olympus IX50 inverted phase contrast microscope is a high quality inverted phase contrast microscope. This microscope comes with a binocular viewing head, and a digital camera for image capture. The microscope comes with phase 4x, 10x, and 20x objectives.


Leica SP5 Confocal
BSB 644C
Contact Ray Deepe

The Leica TCS SP5 Confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. The system provides the full range of scan speeds at the highest resolution. With its proven, high-efficiency SP detection with five channels simultaneously and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID


Leica SP8 Confocal
BSB 644D
Contact Ray Deepe

The SP8 confocal microscope allows you to make proper and detailed observations of fast biological processes. Your experimental work will have the benefit of super-resolution, high-speed imaging, and the capability to image multiple fluorescent markers simultaneously. For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID.

Amira 3D Reconstruction Station #1 and #2
BSB 643A
Contact Ray Deepe

Amira Software is a powerful, comprehensive, and versatile 3D solution for visualizing, analyzing and understanding life science and biomedical research data from many image modalities, including Optical and Electron Microscopy, CT, MRI and other imaging techniques. With incredible speed and flexibility, Amira Software supports advanced bioimaging workflows. From any 3D image data, including time series and multi-channel, Amira Software delivers a comprehensive range of data visualization, processing and analysis capabilities. Amira Software allows life science and biomedical researchers to gain invaluable insights into their data, at different scales and from any modality. For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID.

Color Qube 8580 Printer
BSB 643
Contact Ray Deepe

The ColorQube 8580 color printer by Xerox is a powerful, no-fuss and waste-conscious printing solution that’s simple and highly productive with the advantage of superior color output.

Epson StylusPro 9880 Poster Printer
BSB 644A
Contact Ray Deepe

A true evolution of photographic printing technology, 44-inch Epson Stylus Pro 9880 professional wide-format printer with enhanced 8-color ink technology, resulting in a final print of uncompromising quality.

RMCB Histology Core

Leica HistoCore AUTOCUT Microtome
BSB 649
Contact Ray Deepe

The new generation of microtomes from Leica Biosystems is built upon our 145+ year heritage of market-leading microtome design. It allows users to select among automated, semi auto-automated, or manual sectioning modes based their personal preferences. For use, sign up with your Net ID at: https://regmed.bookedscheduler.com/Web/schedule.php?sd=2020-04-26

Leica Automated Microtome #2
BSB 648
Contact Ray Deepe

Leica Automated Microtome for routine cutting of embedded tissues and embryos. For use, sign up with your Net ID at: https://regmed.bookedscheduler.com/Web/schedule.php?sd=2020-04-26

Manual Microtome #3
BSB 648
Contact Ray Deepe

Routine cutting of embedded tissues and embryos.

Leica Cryostat CM1800
BSB 649
Contact Ray Deepe

Leica CM1800 Cryostat with Microprocessor control and microprocessor technology ensures easy operation. The Leica CM1800 cryostat features a motorized coarse feed that enables rapid trimming and sectioning of standard and non-standard material. The motorized course feed allows the user to trim from 1 to 60 um with a 59 mm vertical stroke and a 25 mm horizontal specimen feed.

Paraffin Station
BSB 649
Contact Ray Deepe

For use, sign up with your Net ID at the Departmental Booking Scheduler with your NetID.

Oven and Vacuum Oven
BSB 649
Contact Ray Deepe

Ovens for embedding tissues and embryos in paraffin.

RMCB Molecular Analytics

BSB 633 Contact Jamie Barth

Instruments requiring scheduling via Infinity can be accessed through the portal for the Proteogenomics Facility.

• Infinity registration and login: https://secure2.ideaelan.com/secure/public/applogin.aspx
• Once logged in, you can view schedulers by selecting the “Instruments” tab and toggling the facility selector to Proteogenomics
• Request access to the instruments by mousing over the instrument and clicking the access request
• Once approved, you can make reservations by clicking and dragging on the calendar. Note that a UDAK is required to complete the reservation, whether there is a fee charged or not.

BioRad CFX96 Touch real time quantitative PCR machine

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.


BioRad CFX96 Connect real time quantitative PCR machine

The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.


BioRad PX1 plate sealer

The PX1 PCR plate sealer provides consistent and uniform sealing across an entire microplate. This semiautomated heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes human variability from plate sealing and minimizes sample evaporation from the PCR workflow. This versatile instrument is compatible with a variety of heat-sealing films and foils and a wide range of PCR plates.

NanoDrop One C spectrophotometer

Prevent costly delays with an enhanced understanding of sample quality using the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometers. Innovative Thermo Scientific™ Acclaro™ Sample Intelligence technology is built into every instrument for improved measurement accuracy and contaminant identification. Quantify and qualify DNA, RNA, and protein samples in seconds with only 1-2 µL, and obtain full-spectral data before you decide to use samples in downstream applications. Additional cuvette position also available.

Qubit 2.0 fluorometer

Accurate and sensitive nucleic acid and protein quantitation demands the highest levels of performance, from the analyte-specific assay to the analytical instrument. The Qubit 2.0 Fluorometer is Life Technologies’ second-generation desktop fluorometer designed to work seamlessly with our highly selective Qubit DNA, RNA, and protein assays. Together, they form the Qubit Fluorometric Quantitation Platform, which delivers unprecedented accuracy and sensitivity through sophisticated and proven fluorescence-based technologies.

Qubit 3.0 fluorometer

The Qubit 3 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive fluorescence-based Qubit quantitation assays. The fluorescent dyes used in these assays emit signals only when bound to specific target molecules, even at low concentrations, thus minimizing the effects of contaminants, including degraded DNA or RNA. The integrated design of the instrument and assays results in quantitation that is far more sensitive than UV absorbance, making this system essential for quantitation of precious samples (samples that are rare, difficult to purify, or expensive to either obtain or prepare) or samples for 'delicate' applications (samples for downstream assays that are extremely sensitive to sample conditions).

Agilent Biotek Synergy H1 Hybrid Multi Mode Plate Reader

BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.

Perkin Elmer GeneAmp 9700 Thermal Cycler

The Perkin Elmer GeneAmp 9700 PCR Thermo Cycler system is intended to be used for high-sample-volume polymerase chain reaction (PCR) applications including DNA and RNA. Users can take advantage of oil-free operation with this model’s heated lid and Thermal Isolation Frame. The graphical user interface of this Perkin Elmer model enhances user experience and provides clear instructions for hassle-free programming and manipulation.

MJ Dyad thermal cycler

The MJ Research Dyad PTC-220 is a fast and efficient PCR System, with two 96-well blocks. This thermal cycler is modular, consisting of a dual bay chassis and easy to change thermal blocks that make it easy to accommodate multiple protocols and multiple users. This PCR thermal cycler has a temperature range of -5 to 105°C, with a ramping speed of 3°C per second.

Monolight 2010C luminometer

Basic luminometer used to measure luciferase activity etc.

Agilent 2100 Bioanalyzer

The Bioanalyzer is a chip-based capillary electrophoresis machine to analyse RNA, DNA, and protein. It is produced by Agilent and widely used, among other things, in RNA quality control measurements.

BluePippin nucleic Acid size selection system

The BluePippin is a DNA size selection system from Sage Science. DNA size selection for next generation sequencing allows for separation from 100 bp – 50 kb using a pulsed-field electrophoresis.
The BluePippin systems use pre-cast and disposable agarose gel cassettes. DNA fractions are collected by electro-elution into a buffer-filled well using a branched channel configuration with switching electrodes. The timing of switching is determined by measuring the rate of DNA migration with optical detection of labelled markers. The position of the detectors beneath the gel cassette is shown in the schematic below.

Affymetrix DNA MicroArray System

GeneChip Scanner 3000 7G, 640 hybridization oven, two FS450 fluidics workstations

Diagenode Biorupter Ultrasonicator

Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.

Flow Bioinformatics Software

Partek® Flow® is specifically for the compute intensive needs of next generation sequencing applications with flexible installation and user management options. It is a start-to-finish software analysis solution for next generation sequencing data applications. It has an easy-to-use interface, robust statistical algorithms, information-rich visualizations, and cutting-edge genomic tools enabling researchers of all skill levels to confidently perform data analysis. For use, first contact Dr. Jamie Barth who will provide a basic introduction, then sign up with your Net ID at the Departmental Booking Scheduler with your NetID..