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MUSC Proteomics Center

The MUSC Proteomics Center is focused on clinical and translational proteomics and development of centralized diagnostics. It is comprised of independent investigators, two core laboratories (Mass Spectrometry Facility and MS Imaging Research Center), and supported by external funding, as well as the MUSC Provost Office, College of Medicine, Hollings Cancer Center, NIH-COBRE, and SmartState funding. Mass spectrometry is the key analytical method for proteomics research, and the Center has recently obtained significant upgrades to an extensive mass spectrometry facility. Current instrumentation includes three ion trap instruments (one Thermo Orbitrap Elite with ETD, two Thermo LTQ's) for analysis of post-translational modifications like phosphorylation and glycosylation. Quantitative proteomics of cultured cells using SILAC and iTRAQ/TMT labeling, as well as characterization of multiple types of post-translational modifications, is the primary emphasis for use of the Orbitrap. Also in the facility are a dual-source Bruker Solarix 7T Fourier Transform-ICR mass spectrometer and two Autoflex MALDI-TOF instruments used primarily for MALDI tissue imaging. The MALDI tissue imaging approaches are histopathology directed, and used for two-dimensional spatial profiling of proteins, lipids, glycans, metabolites, and small molecule drugs. The Center leverages high-performance computing resources with modern protein search algorithms, mass spectrometry based quantification analysis, statistical analysis (frequentist or Bayesian techniques), data-driven systems biology, -omic integration techniques, and classifier development (machine learning based) to generate actionable and hypothesis driving results.

 Key Personnel and Contact Info

Director, MUSC Proteomics Center

Richard R. Drake, Ph.D.

843-792-4505

draker@musc.edu

Director, MSC Imaging Research Center

Peggi Angel, Ph.D.

843-792-8410

angelp@musc.edu

 

Director, Mass Spectrometry Facility

Lauren E. Ball, Ph.D.

843-792-4513

ballle@musc.edu

Lab Manager, Mass Spectrometry Facility

Jennifer Bethard, MS

843-792-8637

bethard@musc.edu

 

For information about mass spectrometry imaging capabilities in the MS Imaging Research Center, contact Dr. Peggi Angel (angelp@musc.edu) or Dr. Richard Drake (draker@musc.edu).

For information about exosomes obtained from clinical specimens and N-linked glycosylation profiling, contact Dr. Richard Drake (draker@musc.edu).

For information about computational proteomics and bioinformatics approaches, contact Dr. Anand Mehta (mehtaa@musc.edu).